Focusing on cell adhesion molecules of the integrin family and their signaling pathways, the group “Molecular and Cellular Radiobiology” has made considerable contributions to the molecular understanding of cell adhesion-mediated radioresistance (CAM-RR) and cell adhesion-mediated drug resistance (CAM-DR).
Signaling cascades of integrins and their interactions with transmembrane growth factor receptors are involved in the regulation of cell fate upon treatment with anticancer agents. The following scheme delineates a selection of molecules investigated by this research group.
Integrins, their interactions with transmembrane growth factor receptors (Receptor-tyrosine-kinase, RTK) and intracellular signaling and adapter proteins. Scheme delineates a selection of molecules investigated by the research group members of “Molecular and Cellular Radiobiology” (published in Cordes N, Hehlgans S, Eke I. Adhesion, Invasion, Integrins and Beyond. In Medical Radiology - Radiation Oncology. Vol.: The Impact of Tumor Biology on Cancer Treatment and Multidisciplinary Strategies. Eds.: M. Molls, A.J. Giaccia, C. Nieder, M.S. Anscher. Springer-Verlag Heidelberg, 2009).
Main aims of this research group are:
- Understanding the molecular and cellular response of cells to ionizing radiation
- Identification of tumor-specific target molecules critically involved in tumor cell radio- and chemoresistance
- Untangling of the molecular and cell biological mechanisms of how a specific target molecule mediates radio- and/or chemoresistance in tumor cells
- Designing targeting strategies to inhibit these radio/chemosensitivity-related target molecules and translating these approaches from bench to bedside
To achieve the above mentioned goals, a variety of methodologies are performed by this group with a particular focus on more physiological 3D cell culture models:
- Colony formation assay
- Cell proliferation
- SDS-PAGE and Western blotting
- siRNA and gene transfection
- Signal transduction
- 3D growth
- Histology (immunohistochemistry, immunofluorescence)
- Immunoprecipitation and mass spectrometry (in cooperation)
- Proteome- and phosphoproteome analysis (in cooperation)
- DNA microarray analysis (in cooperation)
- Cell migration and invasion