Focusing on cell adhesion molecules of the integrin family and their signaling pathways, the group “Molecular and Cellular Radiobiology” has made considerable contributions to the molecular understanding of cell adhesion-mediated radioresistance (CAM-RR) and cell adhesion-mediated drug resistance (CAM-DR).

Signaling cascades of integrins and their interactions with transmembrane growth factor receptors are involved in the regulation of cell fate upon treatment with anticancer agents. The following scheme delineates a selection of molecules investigated by this research group.

Integrins, their interactions with transmembrane growth factor receptors (Receptor-tyrosine-kinase, RTK) and intracellular signaling and adapter proteins. Scheme delineates a selection of molecules investigated by the research group members of “Molecular and Cellular Radiobiology” (published in Cordes N, Hehlgans S, Eke I. Adhesion, Invasion, Integrins and Beyond. In Medical Radiology - Radiation Oncology. Vol.: The Impact of Tumor Biology on Cancer Treatment and Multidisciplinary Strategies. Eds.: M. Molls, A.J. Giaccia, C. Nieder, M.S. Anscher. Springer-Verlag Heidelberg, 2009).

Main aims of this research group are:

  • Understanding the molecular and cellular response of cells to ionizing radiation
  • Identification of tumor-specific target molecules critically involved in tumor cell radio- and chemoresistance
  • Untangling of the molecular and cell biological mechanisms of how a specific target molecule mediates radio- and/or chemoresistance in tumor cells
  • Designing targeting strategies to inhibit these radio/chemosensitivity-related target molecules and translating these approaches from bench to bedside

To achieve the above mentioned goals, a variety of methodologies are performed by this group with a particular focus on more physiological 3D cell culture models:

  • Colony formation assay
  • Cell proliferation
  • SDS-PAGE and Western blotting
  • siRNA and gene transfection
  • Signal transduction
  • 3D growth
  • Spheroids
  • Histology (immunohistochemistry, immunofluorescence)
  • Apoptosis
  • Immunoprecipitation and mass spectrometry (in cooperation)
  • Proteome- and phosphoproteome analysis (in cooperation)
  • DNA microarray analysis (in cooperation)
  • Cell migration and invasion